Inhibition of CD83 Alleviates Systemic Inflammation in Herpes Simplex Virus Type 1-Induced Behçet’s Disease Model Mouse

Figure 3

CD83 siRNA decreases the frequencies of CD83+ cells in normal mice. (a–d) Frequencies of CD40, CD83, CD80, and CD86 in the surface of peripheral blood leukocytes (PBL) and in the peritoneal macrophages of normal mice treated with CD83 siRNA were evaluated by FACS analysis ( in each group). (e–h) CD83 siRNA decreases frequencies of CD83 in BD mice. Frequencies of CD40, CD83, CD80, and CD86 in the surface of peripheral blood leukocytes (PBL) and in the peritoneal macrophage of BD mice treated with CD83 siRNA were evaluated by flow cytometry analysis. , BD symptomatic mice were used in control groups as scramble siRNA; , BD symptomatic mice were used in the 0.5 μmol and 1.0 μmol CD83 siRNA-treated groups. (i, j) CD83 siRNA treatment significantly decreased the severity score of BD mice and improved symptoms. (k) Treatment schedule of CD83 siRNA to BD mice. (l) Representative histogram of CD83+ cell frequencies in PBL and peritoneal macrophage of BD mice treated with CD83 siRNA. (m–s) Regulatory T cells in BD mice analyzed by FACS analysis. Frequencies of CD4+, CD4+Foxp3+, CD4+CD25+, and CD4+CD25+Foxp3+ regulatory T cells in BDN (), BD (), jetPEI-treated BD (), and CD83 siRNA-treated BD mice () were evaluated by FACS analysis. indicates the number of mice used in each group. The value was determined by Student’s -test. Experiments were performed more than three independent times.

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