Mediators of Inflammation / 2019 / Article / Fig 3

Research Article

Andrographolide Protects against HG-Induced Inflammation, Apoptosis, Migration, and Impairment of Angiogenesis via PI3K/AKT-eNOS Signalling in HUVECs

Figure 3

Andr regulates PI3K/AKT-eNOS signalling in vitro. (a) The expression of P-PI3K, T-PI3K, P-AKT, T-AKT, P-eNOS, and T-eNOS protein in each group (). (b–d) Quantification analysis of p-PI3K/PI3K protein, p-AKT/AKT protein, and p-eNOS/eNOS protein. (e, f) Effects of LY294002 at different concentrations on P-P13K and quantitative analysis of p-PI3K/PI3K protein (). (g, h) Effects of MK-2206 at different concentrations on P-AKT and quantitative analysis of p-AKT/AKT protein (). (i, j) Effects of L-NAME at different concentrations on P-eNOS and quantitative analysis of p-eNOS/eNOS protein (). vs. the control group. # vs. the HG group.

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