Isorhamnetin Inhibits Liver Fibrosis by Reducing Autophagy and Inhibiting Extracellular Matrix Formation via the TGF-β1/Smad3 and TGF-β1/p38 MAPK Pathways
IH inhibited autophagy in fibrotic hepatic tissues. (a) The mRNA expression of LC3 and beclin-1 in liver tissues was analyzed by qPCR. Results are given as fold change over the control (vehicle or sham) group. (b) Protein levels of LC3 and beclin-1 were analyzed by western blotting. (c) Beclin-1 protein expression was analyzed using immunohistochemistry (brown indicates positive staining, original magnification: ×200, scale bar: 100 μm). Data are presented as (, compared to the vehicle (sham) group, compared to the CCl4 (BDL) group, and compared to the CCl4 (BDL)+IH 10 mg/kg group).
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