Inhibition of Cerebral High-Mobility Group Box 1 Protein Attenuates Multiple Organ Damage and Improves T Cell-Mediated Immunity in Septic Rats

Figure 2

Time-dependent effects of an ICV injection of BoxA on multiple organ damage induced during sepsis. (a–e, h, i) Serum biochemical parameters, including creatine kinase (CK), CK-MB, aspartate aminotransferase (AST), alanine aminotransferase (ALT), cholylglycine (CG), blood urea nitrogen (BUN), and creatinine (Cr), were determined using a HITACHI 7600 biochemical analyser as these parameters reflect multiple organ dysfunction. Serum samples were collected at 24 h, 48 h, and 72 h after cecal ligation and puncture (CLP) surgery. A dose of 10 μg BoxA was injected into the left lateral ventricle at 0 h, 24 h, and 48 h after CLP surgery. (f, g) The W/D and MPO activities of lung tissues were quantified by weighing and the use of an ELISA kit, respectively. Lungs were harvested and weighed at 24 h, 48 h, and 72 h after CLP surgery. (Each point represents the of three independent experiments, ; vs. the sham group; vs. the sepsis group.)

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