Representative histograms demonstrating integrin CD11b,c/Mac-1, CD11a/LFA-1, and CD49d/VLA-4 expression on peripheral blood leukocyte subsets in Sprague-Dawley rats. Representative dot plots illustrating gating strategy, including exclusion of doublets using forward scatter area (FSC-A) versus forward scatter width (FSC-W) analysis, and gating for lymphocyte, monocyte, and granulocyte subpopulations are shown on (a). In the case of evaluating cytotoxic T cells (CD4-CD8+) and helper T cells (CD4+CD8-), CD3+ lymphocytes were additionally analyzed for CD4 and CD8 expression (b). Granulocytes were defined on the basis of forward scatter area (FSC-A) vs. side-scatter area (SSC-A) (a), and additionally defined as CD3- and His48+ cells (c). B cells were defined as MHCII+ cells among the lymphocyte gated population (d). Representative histograms demonstrating integrin expression during LS and HS diets are shown for CD11b,c/Mac-1 (e), CD11a/LFA-1 (f), and CD49d/VLA-4 (g).