Abnormal Expression of DICER1 Leads to Dysregulation of Inflammatory Effectors in Human Synoviocytes

Figure 2

Altered production of cytokines and chemokines caused by gain or loss of DICER1 function for 48 h in human synoviocytes SW982. (a) Image result of cytokine array detecting conditioned medium from cells transfected with DICER1 or NC siRNA for 48 h. Two membranes were incubated with SW982 cell supernatant transfected by either negative control or DICER1 siRNA for 48 h. (b) Fold change of cytokine expression in conditioned medium of the cell treated by DICER1 siRNA for 48 h was detected by using cytokine array (quantitative result of (a)). Cytokines with fold change beyond ±1.5 were shown. (c) ELISA assay result for IL-8, TGF-β1, and TIMP1 cytokine expression in supernatant of SW982 cells with DICER1 KD or overexpression. Bar: from three independent transfection experiments. Triplicates were used for each transfection experiment, and duplicates were used in ELISA assay. using the Mann-Whitney test. (d) IPA predicted top 5 diseases and biofunctions related to Dicer1 deficiency-induced altered production of cytokines and chemokines.

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