hucMSCs Attenuate IBD through Releasing miR148b-5p to Inhibit the Expression of 15-lox-1 in Macrophages

Figure 3

hucMSCs attenuate the IBD through regulating 15-lox-1 expression in macrophages. (a) The model group was designed to contain the control, DSS, GFP-DSS+MSC, 15-lox-1-DSS+MSC, and 15-lox-1-DSS+DT+MSC groups. Adenovirus of GFP/15-lox-1 was given 2 days prior to DSS treatment, and DT was given 1 day prior to DSS treatment. (b) The body weight loss and (c) the DAI of each group are presented. (d) The colon lengths of each group are presented. (e) HE of colon tissues of each group is presented (100x, ). (f) IHC of PCNA expression in the colon tissues of each group is presented (100x, ). (g) The inflammatory cytokines (IL-1β, IL-6, and TNF-α) and 15-lox-1 expression of colon tissues were measured via QRT-PCR analyses. (h) The expression of PCNA, caspase-3, 15-lox-1 and β-actin proteins in the col8on tissues was measured by Western blot. for each group. Data shown were representative of three independent experiments. Data represent . , , and by ANOVA.