hucMSCs Attenuate IBD through Releasing miR148b-5p to Inhibit the Expression of 15-lox-1 in Macrophages

Figure 5

miR148b-5p from hucMSCs attenuates the IBD through downregulated 15-lox-1 expression in vivo. (a) The model group was designed to contain the control, DSS, and DSS+MSC transfected with the miR148b-5p inhibitor, negative control, or miR148b-5p mimic groups. (b) The body weight loss and (c) the DAI of each group are presented. (d) The colon lengths of each group are presented. (e) HE of colon tissues of each group is presented (100x, ). (f) IHC of PCNA expression in the colon tissues of each group is presented (100x, ). (g) The inflammatory cytokines (IL-1β, IL-6, and TNF-α) and miR148b-5p and 15-lox-1 expression of colon tissues were measured via QRT-PCR analyses. (h) The expression of PCNA, caspase-3, 15-lox-1, and β-actin proteins in the colon tissues was measured by Western blot. for each group. Data shown were representative of three independent experiments. Data represent the . , , and by ANOVA.