The Influence of Photoperiod on the Action of Exogenous Leptin on Gene Expression of Proinflammatory Cytokines and Their Receptors in the Thoracic Perivascular Adipose Tissue (PVAT) in Ewes

Table 2

Genes analysed by real-time RT-PCR with Gene Bank accession numbers to gene sequence, full name, abbreviation, amplicon size and location in gene sequence, and primer characteristics.

Gene Bank acc. no.	Gene	Amplicon size (bp) (localization in the gene sequence)	Primer (forward, F; reverse, R) sequence 5→3	Reference
NM_001009763.1	LEPR: leptin receptor	123 (665 (exon 6)–787 (exon 6/7))–encoding all forms of receptor	F: CTGTGCCAACAGCCAAACT; R: GTGGATCAGGCTTCACAACA	Originally designed
NM_001009465.2	IL1B: interleukin-1β	137 (373–509)	F: CAGCCGTGCAGTCAGTAAAA; R: GAAGCTCATGCAGAACACCA	[24]
NM_001206735.1	IL1R1: IL1 receptor, type I	124 (1455–1578)	F: GGGAAGGGTCCACCTGTAAC; R: ACAATGCTTTCCCCAACGTA	[25]
NM_001046210.2	IL1R2: IL1 receptor, type II	161 (981–1141)	F: CGCCAGGCATACTCAGAAA; R: GAGAACGTGGCAGCTTCTTT	[26]
NM_001009392.1	IL6: interleukin-6	165 (361–525)	F: GTTCAATCAGGCGATTTGCT; R: CCTGCGATCTTTTCCTTCAG	[25]
NM_001110785.3	IL6R: IL6 receptor	149 (288–436)	F: TCAGCGACTCCGGAAACTAT; R: CCGAGGACTCCACTCACAAT	[25]
XM_012096909.2	IL6ST: IL6 signal transducer (glycoprotein 130)	139 (573–711)	F: GGCTTGCCTCCTGAAAAACC; R: ACTTCTCTGTTGCCCACTCAG	[27]
NM_001024860.1	TNFA: tumour necrosis factor	153 (426–578)	F: CAAATAACAAGCCGGTAGCC; R: AGATGAGGTAAAGCCCGTCA	[25]
NM_001166185.1	TNFR1 (TNFRSF1A): TNF receptor, type I (TNF superfamily member 1A)	137 (310–446)	F: AGGTGCCGGGATGAAATGTT; R: CAGAGGCTGCAGTTCAGACA	[25]
NM_001040490.2	TNFR2 (TNFRSF1B): TNF receptor, type II (TNF superfamily member 1B)	122 (772–893)	F: ACCTTCTTCCTCCTCCCAAA; R: AGAAGCAGACCCAATGCTGT	[25]
NM_001190390.1	GAPDH: glyceraldehyde-3-phosphate dehydrogenase	143 (135–258)	F: TGACCCCTTCATTGACCTTC; R: GATCTCGCTCCTGGAAGATG	[24]
NM_001009784.2	ACTB: beta actin	122 (426–547)	F: GCCAACCGTGAGAAGATGAC; R: TCCATCACGATGCCAGTG	[26]
XM_004005023.3	HDAC1: histone deacetylase 1	115 (722–836)	F: CTGGGGACCTACGGGATATT; R: GACATGACCGGCTTGAAAAT	[25]

Primers designed using Primer3web version 4.0.0 (http://bioinfo.ut.ee/primer3/).