Relationship between Helicobacter pylori Infection and Plasmacytoid and Myeloid Dendritic Cells in Peripheral Blood and Gastric Mucosa of Children

Figure 2

Gating strategy for multicolor flow cytometric detection of total dendritic cells and their subsets. At first, leukocytes were gated by forward scatter characteristic (FSC) versus side scatter characteristics (SSC). Next, doublets and aggregates were avoided by selecting singlet cells on a forward scatter: FSC (area)/FSC (height) plot. Then total dendritic cells (tDCs) were gated for fluorescence intensity of HLA-DR peridinin-chlorophyll proteins (PerCP) versus lineage-1 fluorescein (FITC), and using the fluorescence intensity of CD11c phycoerytrin (PE) or CD123 PE, myeloid dendritic cells (mDCs) or plasmacytoid dendritic cells (pDCs) were gated (open histrograms). Then in tDCs and their subsets, a percentage of CD83⁺ and CD86⁺ cells was gated (open histograms.) The grey line represents the staining profile for fluorescence minus one (FMO) control.