Research Article | Open Access
Sorrel Extract Reduces Oxidant Production in Airway Epithelial Cells Exposed to Swine Barn Dust Extract In Vitro
Exposure to hog barn organic dust contributes to occupational lung diseases, which are mediated by inflammatory and oxidative stress pathways. Isoprostanes—a family of eicosanoids produced by oxidation of phospholipids by oxygen radicals—are biomarkers of pulmonary oxidative stress. Importantly, 8-isoprostane has been implicated as a key biomarker and mediator of oxidative stress because it is a potent pulmonary vasoconstrictor. Antioxidants found in fruits and vegetables hold promise for preventing or reducing effects of oxidative stress-related diseases including chronic bronchitis and chronic obstructive pulmonary disease (COPD). Here, we investigated 8-isoP and oxidant production by organic dust-exposed airway epithelial cells and the inhibitory effects of an extract from calyces of the sorrel plant, Hibiscus sabdariffa, on oxidant-producing pathways. Confluent cultures of normal human tracheobronchial epithelial cells were pretreated or not with 1% sorrel extract prior to 5% dust extract (DE) exposure. Following DE treatments, live cells, cell-free supernatants, or cell extracts were evaluated for the presence of 8-isoprostane, superoxide, hydrogen peroxide, nitric oxide, hydroxyl radical, peroxynitrite, and catalase activity to evaluate sorrel’s inhibitory effect on oxidative stress. The well-known radical scavenging antioxidant, N-acetyl cysteine (NAC), was used for comparisons with sorrel. DE exposure augmented the production of all radicals measured including 8-isoprostane ( value < 0.001), which could be inhibited by NAC or sorrel. Among reactive oxygen and nitrogen species generated in response to DE exposure, sorrel had no effect on H2O2 production and NAC had no significant effect on NO⋅ production. The observations reported here suggest a possible role for sorrel in preventing 8-isoprostane and oxidant-mediated stress responses in bronchial epithelial cells exposed to hog barn dust. These findings suggest a potential role for oxidative stress pathways in mediating occupational lung diseases and antioxidants within sorrel and NAC in reducing dust-mediated oxidative stress within the airways of exposed workers.
Respiratory diseases such as asthma, chronic bronchitis, and chronic obstructive pulmonary disease (COPD) are known to increase with concentrated animal feeding operation (CAFO) exposures . Exposure to animal husbandry dusts, such as organic dust from swine production buildings, is the leading cause of pulmonary disease in these professions. Data collected on farm workers from Iowa and North Carolina from 2005 to 2010 reported an increase of wheezing, coughing, and excessive phlegm . Rodriquez et al. observed that California farmers that worked more years had a higher likelihood of a worse forced expiratory volume (FEV) in 1 second divided by FEV in 6 seconds (FEV1/FEV6) ratio . Current therapeutic measures do not adequately address symptoms in this population .
Many of these occupational lung diseases are mediated by proinflammatory cytokines including interleukin-6 (IL-6) and IL-8, whose levels are known to increase in respiratory cells and tissues following swine dust exposure . IL-8 is a potent recruiter of neutrophils and other granulocytes, and it promotes phagocytosis, a process that contributes to oxidative stress by releasing free radicals to neutralize bacteria. Regardless of the source, free radicals mediate injury by binding to and inhibiting the function of important macromolecules such as proteins, lipids, and DNA. Isoprostanes are prostaglandin-like compounds formed by reaction of free radicals with arachidonic acid in phospholipids and are recognized as biomarkers of oxidative stress [6–9]. Measurement of isoprostanes in various biological fluids is used for assessing oxidative stress in healthy subjects and patients with respiratory diseases including asthma and COPD [10–12]. Inhibition of oxidative stress mechanisms may reduce subsequent airway inflammation.
Phytonutrients are bioactive compounds found in plants that promote health and strengthen immunity . Studies that analyze the anti-inflammatory properties of dietary products and their effects on hog barn dust-mediated inflammation have been conducted. Vitamin D was reported to inhibit organic dust-induced proinflammatory cytokines in vitro (human airway epithelial cells and monocytes) and ex vivo (mouse lung slices) . Mice exposed to hog barn dust but allowed to consume Moringa tea had fewer white blood cells present in bronchoalveolar lavage than mice only drinking water . Hibiscus sabdariffa (sorrel) of the Malvaceae family is a plant native to the West Indies, Jamaica, and China and has been grown in the United States. Sorrel is consumed in foods such as tea, jams, and jellies worldwide . Sorrel, also known as roselle, has been studied for its antimicrobial, anti-inflammatory, and antioxidant capacity [17–20]. Sorrel calyces contain potent phytonutrients that are rich in antioxidants and contain high anthocyanin levels .
The purpose of this study was to determine if pretreatment with sorrel will reduce swine facility dust-mediated oxidative stress. Primary airway tracheobronchial epithelial cells were exposed to a dust extract in vitro, and endpoint assays measured production of intracellular oxygen radicals and detection of oxidative stress biomarkers by airway epithelial cells in vitro. Results of our studies showed that pretreatment with sorrel reduced free radical production by airway cells that were exposed to swine facility dust extract. Sorrel’s antioxidant action was similar to the well-known radical scavenger, NAC. Taken together, these data reveal previously uncharacterized antioxidant properties of sorrel on airway cells exposed to swine facility dust extract and provide support for future in vitro studies to test effectiveness of sorrel as a potential dietary supplement to prevent swine dust-mediated lung inflammation.
2. Materials and Methods
2.1. Cell Culture
Normal human bronchial epithelial (NHBE) cells (CC-2541, Lonza, Walkersville, MD) were cultured in NHBE complete media (1 : 1 mixture of BEBM/DMEM, Lonza SingleQuots®, and Nystatin) and maintained in incubators at 37°C with a humidified air conditioner with 5% CO2. Cells were used at passage 2 and seeded on rat tail collagen-coated tissue culture plates at a density of cells/cm2 or until confluent. Prior to experimental investigations and after cells reached optimal confluency, cells were switched to a nonstimulatory medium that did not contain epidermal growth factor (EGF-NHBE) or serum overnight (i.e., 23 hours ahead of pretreatments). Antioxidant pretreatments were for one hour as described below.
2.2. Sorrel Phenolic Extraction and Pretreatment
Dried sorrel calyces were purchased from a local grocery store and freeze-dried. The freeze-dried calyces were grounded to powder and then stored at -20°C until use. The phenolics in powdered freeze-dried calyces were methanol extracted by the ultrasound-assisted method as was previously described . To determine the optimal dose of sorrel to be used with dust extract (DE), NHBE cells were incubated with 0-5% sorrel phenolic extracts diluted in culture media for one hour followed by a 1-hour exposure to DE (0-10%). ROS generation in response to sorrel and/or DE gradients was measured using the 2,7-dichlorofluorescein diacetate (DCF) assay (Molecular Probes, Eugene, OR), according to the manufacturer’s instructions. Briefly, NHBE cells were loaded with 10 μM DCF in phenol red-free media for 50 minutes at 37°C, rinsed twice with phosphate-buffered saline, and treated as described. DCF fluorescence was read at 485 nm excitation and 538 emission. Figure 1 summarized dose-response curves and indicates that lower sorrel concentrations (i.e., 1% or 2.5%) did not increase ROS generation when combined with DE (Figure 1(a)). As shown in Figure 1(b), when NHBE cells were pretreated with 0-5% sorrel for one hour prior to exposure to 1% DE for one hour, there was not a significant increase in ROS. Therefore, the sorrel phenolic extract was diluted to 1% sorrel in cell culture medium for a one-hour pretreatment prior to dust extract exposure.
2.3. N-Acetyl-L-cysteine (NAC) Pretreatment
NAC is a potent free radical scavenging compound with antioxidant activity. For pretreatments, 20 mM NAC (Sigma-Aldrich, St. Louis, MO) was prepared in culture media and used to pretreat cells for one hour. This concentration was chosen based on a study from Ye et al. , where the authors utilized a 20 mM NAC concentration to decrease nanotube-mediated ROS generation in A549 lung epithelial cells .
2.4. Preparation of Swine Confinement Facility Dust Extract (DE)
Settled dust was collected from raised surfaces within the swine confinement facility at North Carolina A&T State University, and DE was prepared by dissolving dust (1 g) in HBSS, centrifugation, and 0.2 μm filtration as was previously described .
2.5. DE Exposure and Cell-Free Supernatant Collection
Confluent cultures of NHBE cells that were previously switched to nonstimulatory media for 23 hours then pretreated with sorrel or NAC or kept in media for one hour were washed twice with PBS, then stimulated with DE for 7 hours or as indicated. Post DE exposure, supernatant was collected and centrifuged for 5 minutes at 5,000 RPM. After centrifuging, the supernatant was transferred to a clean microcentrifuge tube without disturbing the cell pellet. The supernatants were kept at -20°C (i.e., for 8-isoprostane, ROS). Supernatants were used immediately upon acquisition for NO⋅ assays.
2.6. Measurement of Cell Viability
To determine the cytotoxic effect of the agricultural dusts on the NHBE cells, a lactate dehydrogenase (LDH) assay (Roche Diagnostics) was performed as per the manufacturer’s instructions on the cell-free supernatant. Cell viability was also assessed using a LIVE/DEAD assay (Molecular Probes) according to the instruction manual.
2.7. Proliferation Assay
Cells were cultured in a clear 96-well plate until they reached 75% confluency. Cells were then pretreated or not with either NAC or sorrel, stimulated with DE, and analyzed using the bromodeoxyuridine/5-bromo-2-deoxyuridine (BrdU) cell proliferation assay (Roche Diagnostics) as per the manufacturer’s instructions.
2.8. Measurement of Superoxide Production by Mitochondria
MitoSOX Red permeates live cells, selectively targets mitochondria where superoxide (O2⋅-) are generated, but not other ROS, and rapidly oxidizes them into a highly fluorescent product . Cells were pretreated or not with antioxidants for one hour and DE for 7 h, washed, and stained with MitoSOX Red at 37°C for 60 minutes, and then, O2⋅- action within cells was measured within 3 hours at 37°C. Cells were imaged using an EVOS FL microscope (AMG) according to the manufacturer’s instructions. Relative fluorescence intensity was measured using ImageJ software.
2.9. Detection of Hydrogen Peroxide, Hydroxyl Radical, and Peroxynitrite
To measure production of reactive molecules hydroxyl radical (⋅OH) and peroxynitrite (ONOO-) produced by DE-exposed cells, a hydroxyphenyl fluorescein (HPF) assay kit was used. For hydrogen peroxide (H2O2) and peroxidase activity assessment, the Amplex Red assay was used. For both assays, the procedures and excitation and emission settings directed by the manufacturer (Molecular Probes) were followed. ImageJ software was used to measure fluorescence intensity.
2.10. Nitric Oxide Production
To measure nitric oxide (NO⋅) production, cell-free supernatants from exposed cells were evaluated using a Griess reagent assay (Promega) according to the manufacturer’s instructions.
2.11. Measurement of Catalase Activity
To measure catalase activity in the NHBE cell cultures exposed to DE in combination with antioxidant pretreatments or none, a catalase activity assay was performed according to the manufacturer’s instruction (Cayman Chemical, Ann Arbor, Michigan) and absorbance was read at 540 nm using a SpectraMax M5 microplate reader.
2.12. Measurement of 8-Isoprostane
To evaluate oxidative stress, an 8-isoprostane (8-isoP) EIA kit (Cayman Chemical, Ann Arbor, MI) was used on cell supernatants of exposed cells as per the manufacturer’s instructions. Supernatants were either diluted 1 : 10 or left undiluted.
2.13. Statistical Analysis
For cell viability studies (LDH and proliferation), one-way analysis of variance followed by Bonferroni posttest for normally distributed data was used. To determine the effect of antioxidant pretreatment on oxidant production following DE exposure, two-way analysis of variance with Bonferroni posttest correction for normally distributed data was used to compare means of control/treatment groups (i.e., media only or DE exposure) and antioxidant groups (i.e., antioxidants with media or antioxidants with DE). Each experiment was conducted at least three independent times (). For all analyses, a two-sided significance level of 0.05 was used.
3.1. Effect of Sorrel Phenolic Extracts on ROS Generation by NHBE Cells
To determine the concentration of sorrel phenolic extract for use in combination with DE, NHBE cells were incubated with 0-5% sorrel for one hour followed by exposure to DE (0-10%) for one hour. Since this study focused on oxidant production, we were interested in knowing if sorrel would induce ROS production. Therefore, NHBE cells were loaded with the nonfluorescent cell-permeant 2,7-dichlorodihydrofluorescein diacetate, which becomes the highly fluorescent DCF compound when the acetates are cleaved by intracellular esterases and oxidation. Lower concentrations of sorrel phenolic extracts, specifically 1% and 2.5%, did not cause ROS generation by NHBE cells exposed to sorrel only or cells pretreated with sorrel and exposed to increasing concentrations of DE (Figures 1(a) and 1(b)). However, increasing DE concentration caused a dose-dependent increase in ROS production by NHBE cells. It was determined that, when combined, lower concentrations of sorrel (1%) and DE (1%) would provide adequate information about DE-regulated ROS production by NHBE cells. While the DCF assay is a good measure for overall ROS production, additional studies were conducted with assays specific for various types of ROS; results are described below.
3.2. Cytotoxicity Levels Increased in NHBE Cells following Exposure to Swine Barn Dusts
Lactate dehydrogenase (LDH) is an enzyme that normally resides in the cytoplasm of the cell. When the plasma membrane is damaged, LDH can be released into the surrounding environment. This leakage can then be used as a marker of cell viability since the level of LDH in culture media is directly proportional to the level of cytotoxicity. In this study, cytotoxicity levels of DE were evaluated and the results are shown in Figure 2(a). A dose-dependent increase of LDH release was observed in cells exposed to hog barn 1-8% DE.
3.3. Effect of DE Exposure on Cell Viability and Proliferation
To assess cell vitality during experimental treatments, viability and proliferative ability assessments were conducted during treatments with live cells. Lower DE concentrations (i.e., 1-5%) did not alter proliferation. However, Figure 2(b) showed higher DE levels—8%—which resulted in a 25% reduction in cell proliferation compared to the control, although this did not reach formal significance. Figure 2(c) shows cells stained with LIVE/DEAD stain and DAPI. The treatment conditions did not affect cell viability as denoted by the limited number of red (i.e., RFP) fluorescent cells. Thus, the 5% DE concentration was selected for subsequent experiments because it initiated a response, i.e., LDH release, in cells that was nontoxic, i.e., no appreciable decrease in cell proliferation or cell viability.
3.4. Hog Barn Dust Increases ROS and RNS Production by NHBE Which Is Reduced by Sorrel
To further determine the effect of animal husbandry dusts on cellular responses, intracellular production of peroxynitrite and hydroxyl radical accumulation were examined. In Figure 3, NHBE cells were exposed to 5% DE for seven hours following pretreatment with media, 20 mM NAC, or 1% sorrel, then stained with MitoSOX to detect superoxide or HPF to detect ROS/RNS (i.e., hydroxyl radical and peroxynitrite). As shown in Figure 3(a), mitochondrial superoxide generation increased after hog barn DE exposure, which was reduced by sorrel and NAC. Figure 3(b) showed that fluorescence intensity of elevated superoxide levels following 5% DE exposure () was significantly reduced by both NAC and sorrel pretreatments (). Figure 3(c) showed fluorescence intensity for DE-mediated intracellular production of hydroxyl radical and peroxynitrite (). Pretreatment with sorrel and NAC decreased the DE-mediated superoxide, hydroxyl radical, and peroxynitrite production ().
3.5. Pretreatment with Sorrel Attenuates NO⋅ Levels in DE-Exposed NHBE Cells
Nitric oxide is a free radical that is predominant in the respiratory tract and is normally found as a signaling molecule. In this study, the levels of nitric oxide in cells exposed to DE as well as an antioxidant pretreatment of NAC or sorrel were examined. In Figure 4, NHBE cells were pretreated with antioxidants (one hour) prior to 5% swine DE exposure for 7 hours. The 5% DE exposure increased nitric oxide levels compared to the control (). Sorrel pretreatment had low supernatant levels of nitric oxide compared to media+DE-exposed cells whereas NAC did not decrease nitric oxide levels.
3.6. Hog Barn Dust Mediates an Increase of Hydrogen Peroxide in NHBE
To further delineate the ROS involved in hog barn dust inflammation, we stimulated NHBE with 5% DE, with or without sorrel and NAC pretreatment, and with 300 μM hydrogen peroxide (H2O2) as a positive control. Figure 5 demonstrates DE-induced hydrogen peroxide/peroxidase activity in NHBE cells (). NAC reduced hydrogen peroxide/peroxidase activity (); however, sorrel do not affect the hydrogen peroxide/peroxidase activity.
3.7. Catalase Activity Was Increased by DE Exposure
Catalase is an endogenous enzymatic antioxidant that catalyzes the conversion of H2O2 to molecular oxygen and water. Therefore, we were interested to know whether sorrel had an effect on enhancing catalase activity in DE-exposed cells. In Figure 6, NHBE were exposed to media only or 5% DE, with or without NAC (20 mM) and sorrel (1%) pretreatments. Catalase activity was increased in cells exposed to 5% DE compared to media and also sorrel-pretreated cells compared to media (). NAC and sorrel did not affect catalase activity when used as pretreatments prior to DE exposure.
3.8. Levels of 8-Isoprostane Are Increased by Swine Dust, and Sorrel Attenuates 8-Isoprostane Levels
The eicosanoid, 8-isoprostane, is a member of the arachidonic acid pathway and is a well-known mediator of oxidative stress . Therefore, since 8-isoprostane is commonly found in the airway and is a known biomarker for oxidative stress, levels of 8-isoprostane were evaluated in NHBE cells that had been exposed to DE for 7 hours following a one-hour pretreatment with 20 mM NAC or 1% sorrel. The levels of 8-isoprostane were significantly higher in the DE only-treated cells as depicted in Figures 7(a) and 7(b). The antioxidant pretreatments with NAC and sorrel significantly lowered 8-isoprostane levels in DE-exposed cells in a similar manner ().
Hog barn dust exposure induces a myriad of complications on the respiratory epithelium, and facility workers are at risk for development of respiratory symptoms and disease . In Pender et al. , results show that hog barn dust (5 and 10%) induces ROS in a dose-dependent manner in THP-1 cells. Wyatt et al. reported that hog barn dust increased nitric oxide levels in a time-dependent manner in bovine ciliated cells . In our current study, we investigated similar effects of hog barn dust, seeking to define the oxidant species produced and also in the context of reversing the ensuing oxidative stress with naturally occurring antioxidants. Many phytonutrients have immunomodulatory effects, and consuming a diet of foods rich in antioxidants can be beneficial for these types of occupational respiratory exposures. Calyces of Hibiscus spp. contain potent free radical scavenging antioxidants such as polyphenolic acids, flavonoids, and anthocyanins . In this study, we utilized a 5% hog barn dust extract similar to findings by Wyatt et al. . Due in part to the complexity of the dust such as the composition of nanoparticles, endotoxins, and other biophysical components, we observed various types of oxidants being induced . With this knowledge of sorrel’s ability to protect respiratory defenses, we tested our hypothesis that polyphenolic sorrel extracts alleviate hog barn dust-mediated oxidant levels.
Our current investigations indicate that hog barn dust increases oxidant species and these oxidants can be quenched by the use of antioxidants. Upon noting that lactate dehydrogenase levels increase with increasing hog barn dust extract concentration, we conducted fluorescence assays that will identify several reactive oxygen and nitrogen species in the hog barn dust extract-exposed cells. We observed an increase of ROS/RNS in cells exposed to 5% hog barn dust for seven hours. Interestingly, NAC and the Hibiscus sabdariffa (sorrel) polyphenolic extract demonstrated similar reducing actions on oxidant production by DE-exposed NHBE cells. The commercially available antioxidant, acetylcysteine (also known as N-acetyl cysteine, N-acetyl-L-cysteine, or NAC), is a thiol compound that has mucolytic potential and is a direct precursor to reduced glutathione (antioxidant) . NAC is also known to decrease respiratory bursts caused by neutrophils, which can lead to a decrease in free radicals and ultimately reduce oxidative stress [30, 31].
This data is consistent with Pender et al., which demonstrates that hog barn dust induces ROS in vitro . We demonstrate that lactate dehydrogenase (LDH) levels increase in a dose-dependent manner which complements the data that shows that hog barn dust induces 8-isoprostane, a biomarker of lipid peroxidation. Free radicals steal electrons from lipids found in the cell membrane which in turns damages the cell causing LDH to seep out. The 8-isoprostane molecule is known to regulate human airway smooth muscle function and can be upregulated in airway diseases such as asthma as well as environmental exposure . Asthmatic children exhibit higher levels of isoprostanes in their exhaled breath condensate . In the study completed by Janicka et al., there were increased levels of 8-isoprostanes in patients with pulmonary diseases such as asthma and chronic bronchitis compared to healthy individuals . Anokwuru and colleagues reported that phenolic extracts were also capable of inhibiting lipid peroxidation . Nordgren and colleagues provided evidence that maresin-1 could decrease levels of organic dust-induced production of IL-8 and IL-6 by bronchial epithelial cells . Maresin-1 can function as a lipid mediator that can alleviate oxidative stress. Anthocyanins also are capable of antioxidative activity against low-density lipoprotein (LDL) oxidation in RAW264.7 cells . Our 8-isoprostane data are consistent with our observations which show that cells exposed to 8% hog barn dust extract experience reduced proliferation, although not significantly. Cells exposed to higher concentrations of dust could be undergoing too much stress to keep up regular maintenance such as proliferation. Losing cells to lipid peroxidation damage could result in general tissue damage due to cells reducing proliferation and cell death.
To answer which free radical species may have played a role in cell damage, we probed several ROS targets. We observed increased hydrogen peroxide levels with a 5% hog barn dust extract exposure, which lead us to suggest that hydrogen peroxide is acting on the cell membrane and causing the increase of LDH observed in this study. Sorrel extracts did not provide protection against hydrogen peroxide for cells exposed to 5% hog barn dust extract. However, NAC, the commercially available antioxidant used for comparisons, did reduce hydrogen peroxide levels. Due to the common cascade of events in the cell, superoxide dismutase will partition superoxide which results in the formation of hydrogen peroxide. Knowing this, we examined the mitochondrial superoxide anion levels. As expected, 5% hog barn dust extract increased the levels of superoxide in NHBE cells. Sorrel was able to reduce superoxide levels at a level similar to NAC. This is significant because it demonstrates a window that sorrel would be able to provide protection. The modulation of the free radical nitric oxide or NO⋅ was also evaluated. NO⋅ acts as a signaling molecule within the airway and can mediate ciliary beat frequency . We observed increases of NO⋅ in response to 5% DE exposure in vitro. This result falls in line with findings from Gerald et al., that NF-κB activity is increased in BEAS2B cell cultures with a 5% hog barn dust exposure . NF-κB is an upstream transcription factor that codes for the gene inducible nitric oxide synthase (iNOS). It is speculated that hog barn dust-mediated ROS contribute to activation of pathways leading to the translocation of NF-κB since it has been reported that reactive oxygen intermediates can stimulate NF-κB signaling . Pretreatment with NAC did not significantly decrease the levels of NO⋅; however, sorrel attenuated these effects. NO⋅ is also a known regulator of ciliary beating. In Wyatt et al. , exposure to hog barn dust extract altered epithelial beating in bovine ciliated cells in vitro. More studies are warranted to fully understand the significance of oxidants such as NO⋅ in airway cells since NO⋅ is a potent signaling molecule. For example, it is responsible for mediating relaxation of airway smooth cells by modulating calcium , an important signaling molecule and cofactor for numerous enzymatic reactions. Thus, it is plausible that in this study, increased levels of NO⋅ released by agricultural dust-stimulated airway epithelial cells could cause a beneficial effect by promoting the relaxation of airway smooth muscle cells; however, this effect was not investigated.
NO-derived compounds including peroxynitrite are known to cause inflammation, oxidative stress, and activation of metalloproteases and inactivation of antiprotease in COPD. Superoxide rapidly couples with NO⋅ to produce the potent proinflammatory molecule peroxynitrite [40, 41]. The endogenous enzymatic antioxidant SOD partitions superoxide into molecular oxygen or hydrogen peroxide which limits its availability for reacting with NO⋅ and other molecules. However, peroxynitrite formation occurs under normal physiological conditions and in the presence of SOD because the reaction of superoxide with NO⋅ occurs more rapidly than the rate of superoxide dismutation . Peroxynitrite is believed to be responsible for most of the toxic actions of superoxide and NO⋅ . In the present study, we observed enhanced peroxynitrite and hydroxyl radical formation by DE-exposed NHBE cells, which was prevented by sorrel and NAC. This finding is consistent with observations reported by Fischer and Voynow, where pre- or coincubation of A549 cells and NHBE cells with dimethylthiourea (DMTU), a scavenger of peroxynitrite, hydroxyl radical, and other hydroxylated products, reduced neutrophil elastase-induced MUC5AC gene expression and oxidative stress .
An antioxidant-rich diet can provide benefits such as decreasing free radical levels and supplying the body with free radical eliminators. The pretreatment of NHBE cells with sorrel reduced NO⋅ in the sorrel and DE-treated cultures which suggests a protective effect . The phenolic compounds that can be found in sorrel are beneficial to the plant to ward against fungi, viruses, and bacteria. These compounds can also be beneficial to humans; phenols in particular are known to scavenge radicals . The sorrel components could be extremely helpful for inhibiting bacterial growth . Since humans cannot synthesize naturally occurring antioxidants like polyphenols, obtaining them from a diet rich in plants such as sorrel can be advantageous [46, 47]. The sorrel extract that is rich in polyphenols has been reported to be more effective than vitamin C to ward off oxidative stress in tissues . The levels of phenolics are higher than many other active substances from plant products and can be efficient as a cytoprotectant, anti-inflammatory and antibacterial [46, 48]. Gerald et al. provide evidence of Listeria, Salmonella, and Escherichia that could be present in the hog barn dust as well as particles that could be deposited in the airways . The exogenous antioxidant components such as manganese and ascorbic acid are found in the sorrel calyx and could help protect against inflammation and other diseases . The sorrel plant as a whole has the potential to be a beneficial antioxidant source. Keeping organic dust components in mind, the polyphenols within sorrel could be participating in antimicrobial and anti-inflammatory mechanisms, although those actions were not investigated here.
As we expected, catalase activity levels increased when cells were exposed to hog barn dust extract and was consistent with elevated hydrogen peroxide levels when cultured cells were treated with hog barn dust extract. We did not observe significant changes in DE-induced catalase activity when cells were exposed to NAC or sorrel in combination with 5% hog barn dust extract exposure, suggesting that sorrel and NAC may not be interfering with catalase activity. We observed an increase of catalase activity in cells exposed to the polyphenolic compounds from sorrel which is consistent with other reports where antioxidant supplements, such as N(G)-nitro-L-arginine methyl ester (L-NAME), increased activities of catalase and SOD . This data represents the antioxidant role of the extract and, more specifically, the polyphenolic extract containing compounds that encourage antioxidant production. In future studies, longer exposure times (beyond 7 hours) will be used to observe levels of catalase and its return to basal levels post hog barn dust extract exposure. Furthermore, studies aimed at assessing the mechanism of airway inflammation induced by hog barn dust exposure and the anti-inflammatory effects of polyphenolic extracts should also take into consideration and measure neutrophil chemotactic mediators including leukotriene B4 (LTB4) .
In summary, exposure to hog barn dust can increase levels of oxidants in respiratory cells. Our findings suggest that hog barn dust modulates oxidant and antioxidant levels in airway epithelium. We have shown that hog barn dust exposures can intensify oxidant levels, and polyphenolic extracts from Hibiscus sabdariffa can mitigate these levels. We report for the first time that hog barn dust extract mediated superoxide, hydrogen peroxide, and lipid peroxidation in human-derived tracheobronchial epithelium and is reduced by pretreatment with a polyphenolic extract of sorrel calyces. Future studies are warranted to further investigate the molecular mechanisms associated with the regulatory effects of hog barn dust on antioxidants such as catalase. Such observations may have imperative consequences on the health of agricultural workers exposed to hog barn dust, and these data present a possible natural solution for reduction of oxidants and subsequent inflammation.
|ANOVA:||Analysis of variance|
|CAFO:||Concentrated animal feeding operations|
|COPD:||Chronic obstructive pulmonary disease|
|EGF:||Epidermal growth factor|
|FEV1/FEV6:||Forced expiratory volume (FEV) in 1 second divided by FEV in 6 seconds|
|GFP:||Green fluorescent protein|
|L-NAME:||N(G)-Nitro-L-arginine methyl ester|
|NF-κB:||Nuclear factor kappa B|
|NHBE:||Normal human bronchial epithelial|
|RAW264.7:||Macrophage Abelson murine leukaemia virus transformed|
|RFP:||Red fluorescent protein|
|RPM:||Revolutions per minute|
|RNS:||Reactive nitrogen species|
|ROS:||Reactive oxygen species|
|SEM:||Standard error measure.|
The data used to support the findings of this study are available from the corresponding author upon request.
Carresse L. Gerald’s present address is Department of Environmental, Earth and Geospatial Sciences, North Carolina Central University, 1801 Fayetteville Street, Durham, North Carolina 27707, USA. Chakia J. McClendon’s present address is Department of Physiology and Biophysics, Stony Brook University, 100 Nicolls Road, Stony Brook, NY 11794, USA. Contents of this study are solely the responsibility of the authors and do not necessarily represent the official views of the National Institute of Food and Agriculture or the National Institutes of Health.
Conflicts of Interest
The authors declare that there is no conflict of interest.
Chakia J. McClendon and Rohit S. Ranabhat contributed equally to this work.
The authors thank Celestine Murray and Kangyue Xu for the technical assistance in the laboratory. The authors thank Susan J. Henning and the NC TraCS K Scholar seminar group for helpful discussions. This research was funded by the National Institute of Food and Agriculture (grant numbers NCX-255-5-11-120-1 and 2011-38821-30967) and National Institutes of Health (grant number 1UL1TR001111). C.J.M. is currently a postdoctoral fellow supported by the National Institutes of Health (grant number K12-GM-102778).
- S. May, D. J. Romberger, and J. A. Poole, “Respiratory health effects of large animal farming environments,” Journal of Toxicology and Environmental Health. Part B, Critical Reviews, vol. 15, no. 8, pp. 524–541, 2012.
- J. A. Hoppin, D. M. Umbach, S. Long et al., “Respiratory disease in United States farmers,” Occupational and Environmental Medicine, vol. 71, no. 7, pp. 484–491, 2014.
- E. J. Rodriquez, M. T. Stoecklin-Marois, D. H. Bennett, D. J. Tancredi, and M. B. Schenker, “Agricultural work exposures and pulmonary function among hired farm workers in California (the MICASA study),” Journal of Agromedicine, vol. 19, no. 4, pp. 427–436, 2014.
- M. Szczyrek, P. Krawczyk, J. Milanowski, I. Jastrzebska, A. Zwolak, and J. Daniluk, “Chronic obstructive pulmonary disease in farmers and agricultural workers - an overview,” Annals of Agricultural and Environmental Medicine, vol. 18, no. 2, pp. 310–313, 2011.
- C. L. Gerald, D. J. Romberger, J. M. DeVasure et al., “Alcohol decreases organic dust-stimulated airway epithelial TNF-alpha through a nitric oxide and protein kinase-mediated inhibition of TACE,” Alcoholism, Clinical and Experimental Research, vol. 40, no. 2, pp. 273–283, 2016.
- J. D. Morrow, T. M. Harris, and L. Jackson Roberts II, “Noncyclooxygenase oxidative formation of a series of novel prostaglandins: analytical ramifications for measurement of eicosanoids,” Analytical Biochemistry, vol. 184, no. 1, pp. 1–10, 1990.
- J. D. Morrow and L. J. Roberts, “The isoprostanes: unique bioactive products of lipid peroxidation,” Progress in Lipid Research, vol. 36, no. 1, pp. 1–21, 1997.
- L. J. Roberts and J. D. Morrow, “Measurement of F2-isoprostanes as an index of oxidative stress in vivo,” Free Radical Biology & Medicine, vol. 28, no. 4, pp. 505–513, 2000.
- J. A. Lawson, J. Rokach, and G. A. FitzGerald, “Isoprostanes: formation, analysis and use as indices of lipid peroxidation in vivo,” The Journal of Biological Chemistry, vol. 274, no. 35, pp. 24441–24444, 1999.
- G. Santini, N. Mores, R. Shohreh et al., “Exhaled and non-exhaled non-invasive markers for assessment of respiratory inflammation in patients with stable COPD and healthy smokers,” Journal of Breath Research, vol. 10, no. 1, 2016.
- P. Montuschi, P. J. Barnes, and G. Ciabattoni, “Measurement of 8-isoprostane in exhaled breath condensate,” Methods in Molecular Biology, vol. 594, pp. 73–84, 2010.
- P. Montuschi, J. A. Nightingale, S. A. Kharitonov, and P. J. Barnes, “Ozone-induced increase in exhaled 8-isoprostane in healthy subjects is resistant to inhaled budesonide,” Free Radical Biology & Medicine, vol. 33, no. 10, pp. 1403–1408, 2002.
- World Health Organization, “Diet, nutrition and the prevention of chronic diseases,” World Health Organization Technical Report Series, vol. 916, no. i-viii, pp. 1–149, 2003, backcover.
- G. A. Golden, T. A. Wyatt, D. J. Romberger et al., “Vitamin D treatment modulates organic dust-induced cellular and airway inflammatory consequences,” Journal of Biochemical and Molecular Toxicology, vol. 27, no. 1, pp. 77–86, 2013.
- M. Mcknight, J. Allen, J. T. Waterman, S. Hurley, J. Idassi, and R. C. Minor, “Moringa Tea Blocks Acute Lung Inflammation Induced by Swine Confinement Dust Through A Mechanism Involving TNF-α Expression, C-JUN N-Terminal Kinase Activation and Neutrophil Regulation,” American Journal of Immunology, vol. 10, no. 2, pp. 73–87, 2014.
- G. Gradinaru, C. G. Biliaderis, S. Kallithraka, P. Kefalas, and C. Garcia-Viguera, “Thermal stability of Hibiscus sabdariffa L. anthocyanins in solution and in solid state: effects of copigmentation and glass transition,” Food Chemistry, vol. 83, no. 3, pp. 423–436, 2003.
- M. Fullerton, J. Khatiwada, J. U. Johnson, S. Davis, and L. L. Williams, “Determination of antimicrobial activity of sorrel (Hibiscus sabdariffa) on Escherichia coli O157:H7 isolated from food, veterinary, and clinical samples,” Journal of Medicinal Food, vol. 14, no. 9, pp. 950–956, 2011.
- T. O. Fakeye, A. Pal, D. U. Bawankule, and S. P. S. Khanuja, “Immunomodulatory effect of extracts of Hibiscus sabdariffa L. (family Malvaceae) in a mouse model,” Phytotherapy Research, vol. 22, no. 5, pp. 664–668, 2008.
- E. S. Kao, J. D. Hsu, C. J. Wang, S. H. Yang, S. Y. Cheng, and H. J. Lee, “Polyphenols Extracted fromHibiscus sabdariffaL. Inhibited Lipopolysaccharide-Induced Inflammation by Improving Antioxidative Conditions and RegulatingCyclooxygenase-2 Expression,” Bioscience, Biotechnology, and Biochemistry, vol. 73, no. 2, pp. 385–390, 2009.
- K. L. Higginbotham, K. P. Burris, S. Zivanovic, P. M. Davidson, and C. N. Stewart Jr., “Aqueous extracts of Hibiscus sabdariffa calyces as an antimicrobial rinse on hot dogs against Listeria monocytogenes and methicillin-resistant Staphylococcus aureus,” Food Control, vol. 40, pp. 274–277, 2014.
- L. G. Maciel, M. A. V. do Carmo, L. Azevedo et al., “Hibiscus sabdariffa anthocyanins-rich extract: chemical stability, in vitro antioxidant and antiproliferative activities,” Food and Chemical Toxicology, vol. 113, pp. 187–197, 2018.
- M. Verghese, S. Ogutu, M. S. C. Fullerton et al., “Determination of antioxidant contents in red sorrel and its nticarcinogenic potential in azoxymethane-induced colonic aberrant crypt foci,” Research Journal of Phytochemistry, vol. 2, no. 2, pp. 69–76, 2008.
- S. F. Ye, Y. H. Wu, Z. Q. Hou, and Q. Q. Zhang, “ROS and NF-κB are involved in upregulation of IL-8 in A549 cells exposed to multi-walled carbon nanotubes,” Biochemical and Biophysical Research Communications, vol. 379, no. 2, pp. 643–648, 2009.
- M. R. C. Pender, S. L. Hurley, D. R. Conklin, and J. T. Waterman, “Exposure to swine housing dust modulates macrophage morphology and function,” American Journal of Immunology, vol. 10, no. 1, pp. 35–45, 2014.
- K. M. Robinson, M. S. Janes, M. Pehar et al., “Selective fluorescent imaging of superoxide in vivo using ethidium-based probes,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 41, pp. 15038–15043, 2006.
- P. Montuschi, M. Corradi, G. Ciabattoni, J. Nightingale, S. A. Kharitonov, and P. J. Barnes, “Increased 8-isoprostane, a marker of oxidative stress, in exhaled condensate of asthma patients,” American Journal of Respiratory and Critical Care Medicine, vol. 160, no. 1, pp. 216–220, 1999.
- T. A. Wyatt, J. H. Sisson, S. G. Von Essen, J. A. Poole, and D. J. Romberger, “Exposure to hog barn dust alters airway epithelial ciliary beating,” The European Respiratory Journal, vol. 31, no. 6, pp. 1249–1255, 2008.
- C. Gerald, C. McPherson, T. M. McDaniel et al., “A biophysiochemical analysis of settled livestock and poultry housing dusts,” American Journal of Agricultural and Biological Sciences, vol. 9, no. 2, pp. 153–166, 2014.
- R. F. Lemanske Jr. and W. W. Busse, “Asthma: clinical expression and molecular mechanisms,” The Journal of Allergy and Clinical Immunology, vol. 125, no. 2, pp. S95–102, 2010.
- I. Beck-Speier, N. Dayal, E. Karg et al., “Oxidative stress and lipid mediators induced in alveolar macrophages by ultrafine particles,” Free Radical Biology & Medicine, vol. 38, no. 8, pp. 1080–1092, 2005.
- I. Beck-Speier, E. Karg, H. Behrendt, T. Stoeger, and F. Alessandrini, “Ultrafine particles affect the balance of endogenous pro- and anti-inflammatory lipid mediators in the lung: in-vitro and in-vivo studies,” Particle and Fibre Toxicology, vol. 9, no. 1, p. 27, 2012.
- L. G. Wood, P. G. Gibson, and M. L. Garg, “Biomarkers of lipid peroxidation, airway inflammation and asthma,” The European Respiratory Journal, vol. 21, no. 1, pp. 177–186, 2003.
- J. A. Voynow and A. Kummarapurugu, “Isoprostanes and asthma,” Biochimica et Biophysica Acta (BBA) - General Subjects, vol. 1810, no. 11, pp. 1091–1095, 2011.
- M. Janicka, A. Kot-Wasik, J. Kot, and J. Namiesnik, “Isoprostanes-biomarkers of lipid peroxidation: their utility in evaluating oxidative stress and analysis,” International Journal of Molecular Sciences, vol. 11, no. 11, pp. 4631–4659, 2010.
- C. Prosper An, I. Esiaba, O. Ajbaye, and A. O. Adesuyi, “Polyphenolic content and antioxidant activity of Hibiscus sabdariffa calyx,” Research Journal of Medicinal Plant, vol. 5, no. 5, pp. 557–566, 2011.
- T. M. Nordgren, A. J. Heires, T. A. Wyatt et al., “Maresin-1 reduces the pro-inflammatory response of bronchial epithelial cells to organic dust,” Respiratory Research, vol. 14, no. 1, p. 51, 2013.
- B. Jain, I. Rubinstein, R. A. Robbins, K. L. Leise, and J. H. Sisson, “Modulation of airway epithelial cell ciliary beat frequency by nitric oxide,” Biochemical and Biophysical Research Communications, vol. 191, no. 1, pp. 83–88, 1993.
- A. Oeckinghaus and S. Ghosh, “The NF-κB family of transcription factors and its regulation,” Cold Spring Harbor Perspectives in Biology, vol. 1, p. a000034, 2009.
- U. Mabalirajan, J. Aich, G. D. Leishangthem, S. K. Sharma, A. K. Dinda, and B. Ghosh, “Effects of vitamin E on mitochondrial dysfunction and asthma features in an experimental allergic murine model,” J Appl Physiol, vol. 107, no. 4, pp. 1285–1292, 2009.
- J. S. Beckman, T. W. Beckman, J. Chen, P. A. Marshall, and B. A. Freeman, “Apparent hydroxyl radical production by peroxynitrite: implications for endothelial injury from nitric oxide and superoxide,” Proceedings of the National Academy of Sciences of the United States of America, vol. 87, no. 4, pp. 1620–1624, 1990.
- W. A. Pryor and G. L. Squadrito, “The chemistry of peroxynitrite: a product from the reaction of nitric oxide with superoxide,” The American Journal of Physiology, vol. 268, no. 5, pp. L699–L722, 1995.
- I. Fridovich, “Superoxide radical and superoxide dismutases,” Annual Review of Biochemistry, vol. 64, no. 1, pp. 97–112, 1995.
- R. Radi, “Peroxynitrite, a stealthy biological oxidant,” The Journal of Biological Chemistry, vol. 288, no. 37, pp. 26464–26472, 2013.
- B. Fischer and J. Voynow, “Neutrophil elastase induces MUC5AC messenger RNA expression by an oxidant-dependent mechanism,” Chest, vol. 117, no. 5, pp. 317S–320S, 2000.
- M. Zhang, N. S. Hettiarachchy, R. Horax, A. Kannan, A. Praisoody, and A. Muhundan, “Phytochemicals, antioxidant and antimicrobial activity of Hibiscus sabdariffa, Centella asiatica, Moringa oleifera and Murraya koenigii leaves,” Journal of Medicinal Plants Research, vol. 5, no. 30, pp. 6672–6680, 2011.
- V. Hirunpanich, A. Utaipat, N. P. Morales et al., “Antioxidant effects of aqueous extracts from dried calyx of Hibiscus sabdariffa Linn. (roselle) in vitro using rat low-density lipoprotein (LDL),” Biological & Pharmaceutical Bulletin, vol. 28, no. 3, pp. 481–484, 2005.
- Y. C. Chang, H. P. Huang, J. D. Hsu, S. F. Yang, and C. J. Wang, “Hibiscus anthocyanins rich extract-induced apoptotic cell death in human promyelocytic leukemia cells,” Toxicology and Applied Pharmacology, vol. 205, no. 3, pp. 201–212, 2005.
- C. M. Andre, Y. Larondelle, and D. Evers, “Dietary antioxidants and oxidative stress from a human and plant perspective: a review,” Current Nutrition & Food Science, vol. 6, no. 1, pp. 2–12, 2010.
- P.-J. Tsai, J. McIntosh, P. Pearce, B. Camden, and B. R. Jordan, “Anthocyanin and antioxidant capacity in roselle (Hibiscus sabdariffa L.) extract,” Food Research International, vol. 35, no. 4, pp. 351–356, 2002.
- H. X. Zhang, S. J. Liu, X. L. Tang et al., “H2S attenuates LPS-induced acute lung injury by reducing oxidative/nitrative stress and inflammation,” Cellular Physiology and Biochemistry, vol. 40, no. 6, pp. 1603–1612, 2016.
- P. Montuschi, “Leukotrienes, antileukotrienes and asthma,” Mini Reviews in Medicinal Chemistry, vol. 8, no. 7, pp. 647–656, 2008.
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