Soluble CD14 Enhances the Response of Periodontal Ligament Stem Cells to Toll-Like Receptor 2 Agonists

Figure 1

Effect of sCD14 on the hPDLSC response to TLR-2 agonist Pam3CSK4. Primary hPDLSCs were stimulated with different Pam3CSK4 concentrations (1-1000 ng/ml), in the presence/absence of sCD14 (250 ng/ml). Untreated cells served as the control. After 24 hours of incubation, IL-6, CXCL8, and CCL2 gene expression levels (a) were measured using qPCR. The -axis shows the -fold expression of the target genes compared to the unstimulated control. The corresponding protein levels in conditioned media (b) were determined by ELISA. All data are presented as from five independent experiments using cells from five different donors. value < 0.05 compared to control. # value < 0.05 compared to the appropriate group stimulated in the absence of sCD14.

(a)

(b)