Potential Role of Caveolin-1 in Regulating the Function of Endothelial Progenitor Cells from Experimental MODS Model

Figure 4

Cav-1 regulates the functions of EPCs. (a) Cav-1 mRNA and protein levels in EPCs after Cav-1 siRNA transfection for 48 h. β-Actin was used as internal control. (b) Cell proliferation of EPCs after Cav-1 siRNA transfection determined by MTT assay. (c) Cell migration of EPCs after Cav-1 siRNA transfection assessed by wound healing assay. (d) The angiogenic ability of EPCs after Cav-1 siRNA transfection evaluated by tube formation assay. (e) Cav-1 mRNA and protein levels in EPCs after incubation with proinflammatory factors and indicated inhibitors. β-Actin was used as internal control. (f) The protein expression levels of p-AKT, total AKT, p-eNOS, and total eNOS assessed by Western blot after incubation with proinflammatory factors and indicated siRNA. (g) NO levels after incubation with proinflammatory factors and indicated siRNA were detected by the Griess method. (h) The protein expression levels of p-AKT, total AKT, p-eNOS, and total eNOS assessed by Western blot after incubation with proinflammatory factors and indicated inhibitors. (i) NO levels after incubation with proinflammatory factors and indicated inhibitors were detected by the Griess method. vs. the control group.

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