Mediators of Inflammation / 2019 / Article / Fig 1

Research Article

Pregnancy-Associated Plasma Protein A Induces Inflammatory Cytokine Expression by Activating IGF-I/PI3K/Akt Pathways

Figure 1

Dose-dependent and time-dependent effects of PAPP-A on MCP-1, TNF-α, and IL-6 expressions in RAW264.7 macrophages. (a, c, e) RAW264.7 macrophages were treated with various concentrations of PAPP-A for 24 h, respectively. (b, d, f) Cells were treated with 5 mg/mL BSA for 24 h or with 200 ng/mL PAPP-A for different durations, respectively. (a, b) MCP-1, TNF-α, and IL-6 mRNA expressions were measured by RT-qPCR. (c, d, e, f) MCP-1, TNF-α, and IL-6 protein expressions were measured by Western blot assays. All the results are expressed as the from three independent experiments, each performed in triplicate. vs. baseline, vs. baseline, and vs. baseline.
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