Paneth Cell Ablation Aggravates Pancreatic and Intestinal Injuries in a Rat Model of Acute Necrotizing Pancreatitis after Normal and High-Fat Diet

Figure 1

Dithizone depletes lysozyme in ileal crypts in rats. Lysozyme protein expression (green) in Paneth cells of the distal ileum as assessed by immunofluorescence. Nuclei were counterstained with DAPI (magnification ×200). Lysozyme mRNA expression as assessed by RT-PCR. (a) STD+saline+ANP vs. STD+DI+ANP. (b) STD+saline+sham infusion vs. STD+DI+sham infusion. (c) HF+saline+ANP vs. HF+DI+ANP. (d) HF+saline+sham infusion vs. HF+DI+sham infusion. , , , , Student’s -test. ANP: acute necrotizing pancreatitis; DI: dithizone; HF: high-fat diet; STD: standard diet. Results were expressed as the ().

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