Melatonin Inhibits Lipopolysaccharide-Induced Inflammation and Oxidative Stress in Cultured Mouse Mammary Tissue

Table 1

Primers used for qPCR.

Genes	Primer sequence (5–3)	Product size (bp)^1	Tm (°C)
L19	Forward: CTGAAGGTCAAAGGGAATGTG	196	60
	Reverse: GGACACAGTCTTGATGATCTC
TNF-α	Forward: AGTCCGGGCAGGTCTACTTT	422	60
	Reverse: GCACCTCAGGGAAGAATCTG
IL-1β	Forward: GGAATGACCTGTTCTTTGAAGTT	345	60
	Reverse: GGCTCCGAGATGAACAACAAAA
IL-6	Forward: CCGGAGAGGAGACTTCACAG	421	60
	Reverse: GGAAATTGGGGTAGGAAGGA
CXCL1	Forward: AAGATGCTAAAAGGTGTCCCCA	389	55
	Reverse: CTCCCACACATGTCCTCACC
MCP-1	Forward: GGTCCCTGTCATGCTTCTGG	236	64
	Reverse: CCTTCTTGGGGTCAGCACAG
RANTES	Forward: ATATGGCTCGGACACCACTC	242	62
	Reverse: GGGAAGCGTATACAGGGTCA
Nrf2	Forward: CTTTAGTCAGCGACAGAAGGAC	227	62
	Reverse: AGGCATCTTGTTTGGGAATGTG
iNOS	Forward: GCATGGACCAGTATAAGGCAAGAC	222	64
	Reverse: GCATGGACCAGTATAAGGCAAGAC
COX-2	Forward: AGACATCCTGATCCTGGTTT	197	60
	Reverse: GTTCAATGGGCTGGAAGACA

¹SYBR qPCR mix allows amplicon size of products in the present study [35, 36]. Abbreviation: L19: ribosomal protein L19 (housekeeping gene); TNF-α: tumor necrosis factor-α; IL: interleukin; CXCL: chemokine CXC motif ligand; MCP-1: monocyte chemotactic protein 1; RANTES: regulated upon activation, normal T-cell expressed and secreted; Nrf2: nuclear factor E2-related factor; iNOS: inducible nitric oxide synthase; COX-2: cyclooxygenase-2.