IL-10 Dampens the Th1 and Tc Activation through Modulating DC Functions in BCG Vaccination

Figure 3

IL-10KO mice showed higher IFN γ production after BCG challenge in the lungs. The mice were treated as described in the legend of Figure 4. The lungs were digested as described in Materials and Methods. The intracellular IFN γ-positive T cells were analyzed by intracellular cytokine staining as described previously [29]. (a) Gating strategy: firstly, the major cells were gated for further gated based on the CD3_Ɛ expression. Then, the CD3_Ɛ⁺ cells were gated on the CD4 and CD8 expression for CD4⁺ and CD8⁺ T cells. (b) The figures from FAC analysis for the IFN-γ⁺ expression were shown. (c) Summary of the percentage of the IFN-γ⁺CD3_Ɛ⁺, CD4⁺, and CD8⁺ T cells in the lungs, and Student’s test was used for analysis. The representative of three independent experiments () with similar results was shown. , .

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