Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells

<div>Validation of mRNA and lncRNA expression by RT-qPCR. The RT-qPCR validation of six mRNAs and lncRNAs. The results showed that the expression levels of the mRNAs FRMD1, SERPINF1, and FEZ1 and lncRNAs RP11-659F24.1, RP11-966I7.4, and LAMB2P1 were upregulated compared with the controls, and the expression levels of the mRNAs PRR21, REG3G, and ACAT2 and lncRNAs ANKRD30BL, CH17-360D5.2, and SOX2-OT were downregulated compared with the controls (a). The results of RT-qPCR were similar to those obtained from the microarray analysis (b).</div>

Mediators of Inflammation

fig4

Figure 4

Figure 4: Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells 

Figure 4 | Identification of the Transcriptional Networks and the Involvement in Angiotensin II-Induced Injury after CRISPR/Cas9-Mediated Knockdown of Cyr61 in HEK293T Cells 