Mediators of Inflammation / 2019 / Article / Fig 1

Research Article

Hydrogen Sulfide Attenuates High Glucose-Induced Human Retinal Pigment Epithelial Cell Inflammation by Inhibiting ROS Formation and NLRP3 Inflammasome Activation

Figure 1

The high-glucose condition decreased cell viability and induced proinflammatory cytokine IL-18 and IL-1β production in ARPE-19 cells. ARPE-19 cells were treated with high or low glucose for 48 hours. (a) Cell viability determined by the CCK-8 assay in the low-glucose group, high-glucose group, high-glucose+H2S group, and high-glucose+NAC group. (b) Protein levels of IL-18 detected by ELISA. (c) Protein levels of IL-1β detected by ELISA. (d) The mRNA expression levels of IL-18 determined by RT-PCR. (e) IL-1β mRNA levels measured by RT-PCR. Data were presented as the error (SE) of three independent experiments. and , compared between the low-glucose and high-glucose groups (low glucose: 5.5 mM, high glucose: 25 mM).

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