Epigenetic Regulation of IL-17-Induced Chemokines in Lung Epithelial Cells

Figure 6

Induction of proinflammatory chemokines is dependent on BET. Human bronchial epithelial cell line HBE1 cells were plated 0.08-0.15 million cells per well in a 12-well plate. After incubating with BronchiaLife™ Epithelial Airway Medium overnight, cells were treated with control medium, 100 ng/ml h-IL-17A protein, 200 nM CPI, and 100 ng/ml h-IL-17A plus 200 nM CPI, respectively. 6 h after stimulation, (a) CXCL1, (b) CXCL2, and (c) CXCL8 mRNA levels were determined by RT-PCR. (d) 24 h after stimulation, supernatant was collected and CXCL8 protein level was measured by ELISA. ; ; ; by a one-way ANOVA test. It is the representative figure from 4 experiments.

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