IL-37 Exerts Anti-Inflammatory Effects in Fetal Membranes of Spontaneous Preterm Birth via the NF-κB and IL-6/STAT3 Signaling Pathway
The expression and distribution of IL-37 in fetal membranes and plasma complicated with spontaneous preterm birth. (a) The plasmas from term () and sPTB delivery women () were collected, and IL-37 level was detected by ELISA. The detection range of IL-37 was 80-20000 pg/ml. (b) The mRNA expression level of IL-37 in fetal membranes was measured by quantitative real-time PCR analysis. The results were normalized to β-actin. (c) The fetal membranes from term () and sPTB delivery women () were harvested, and IL-37 level was detected by western blot. (d) Statistical analysis of western blot in the result (c). (e) Immunofluorescence staining was utilized to locate the expression site of IL-37 (green) in fetal membranes. Scale bar: 200 μm. (f) Immunohistochemical staining of IL-37 in fetal membranes. Scale bar: 200 μm and 100 μm. ;.
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