Comparison of Inhibitor and Substrate Selectivity between Rodent and Human Vascular Adhesion Protein-1

Figure 1

Sequence alignment of recombinant mouse VAP-1 (rmVAP-1), recombinant rat VAP-1 (rrVAP-1), and recombinant human VAP-1 (rhVAP-1). Dark grey highlight indicates the HIS tag. Light grey tag indicates the linker sequence. Amino acids that reside in the active site pocket are highlighted yellow. The amino acids that vary between species are in red or blue. The blue color indicates that the amino acids have side chains with similar properties (nonpolar, polar, acidic, or basic). The amino acids that are bolded are believed to be key for defining the properties of the active site channel. The tyrosine that is converted into topaquinone is highlighted in magenta. The histidine residues that coordinate the copper are highlighted in green.