The Effects of Sidt2 on the Inflammatory Pathway in Mouse Mesangial Cells

Figure 4

Under the stimulation of LPS, the deletion of Sidt2 gene leads to the activation of inflammatory pathway in SV40 MES 13 cells. (a, b) After being treated with LPS for 12 hours, the protein expressions of the NF-κB pathway including IKK β, p-IKK α/β, NF-κB p65, p-NF-κB p65, p-IκBα, IκBα, and TNF-α in the Sidt2^+/+ group, the Sidt2^+/++LPS group, the Sidt2^-/- group, and the Sidt2^-/-+LPS group were detected by western blotting. (c, d) After being treated with LPS for 12 hours, the protein expressions of the JAK/STAT pathway including p-Jak2, p-STAT3, and IL6 in the Sidt2^+/+ group, the Sidt2^+/++LPS group, the Sidt2^-/- group, and the Sidt2^-/-+LPS group were detected by western blotting. (e, f) After being treated with LPS for 12 hours, the protein expressions of the MAPK pathway including p-JNK, p-ERK, p-P38, and ERK in the Sidt2^+/+ group, the Sidt2^+/++LPS group, the Sidt2^-/- group, and the Sidt2^-/-+LPS group were detected by western blotting. , , .

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