Defects of CTLA-4 Are Associated with Regulatory T Cells in Myasthenia Gravis Implicated by Intravenous Immunoglobulin Therapy

Figure 4

IVIg therapy induces Treg and CTLA-4⁺ Treg cells through DCs in MG. Monocyte-derived DCs from MG patients were treated with vehicle, 2 mg/ml or 4 mg/ml IVIg and then cocultured with CD⁴⁺ T cells from PBMC of MG patients for 4 days. Treg cells (CD4⁺CD25⁺FOXP3⁺) and CTLA-4⁺ Treg cells (CD4⁺CD25⁺FOXP3⁺CTLA-4⁺) were then detected by flow cytometry (a, b). At least three independent experiments were performed and statistical significance was determined by test (c, d). Data are expressed as . .

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(b)

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(d)