Research Article
Defects of CTLA-4 Are Associated with Regulatory T Cells in Myasthenia Gravis Implicated by Intravenous Immunoglobulin Therapy
Figure 5
Quantitative analysis of CTLA-4 mRNA in Treg cells from myasthenia gravis patients (MG) and healthy donors (HD). (a) Total RNA was extracted from sorted Treg cells of MG and HD and then real-time PCR was performed to measure CTLA-4 mRNA level. (b) Genomic DNA was prepared from sorted Treg cells and methylation status within CTLA-4 promoter was detected. The methylation percentage within CTLA-4 promoter in Treg cells from HD and MG () was shown. (c) The methylation percentage at -658 and -793 CpGs in Treg cells from MG and MG with IVIg therapy () is shown. Data are expressed as . .
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