The Influence of Adalimumab and Cyclosporine A on the Expression Profile of the Genes Related to TGFβ Signaling Pathways in Keratinocyte Cells Treated with Lipopolysaccharide A
Table 3
Microarray profile of TGFβ-dependent genes in HaCaT culture exposed to LPS or LPS+adalimumab/cyclosporine A ( in the first comparison).
Group
LPS
LPS+adalimumab
LPS+cyclosporine A
H-8 vs C
H-2 vs C
H-8 vs C
H-24 vs C
H-2 vs C
H-8 vs C
H-24 vs C
ID
MRna
203085_s_at
TGFβ1
12.11
4.16
-8.36
-11.07
8.66
-2.33
-1.33
220407_s_at
TGFβ2
4.14
-3.28
-4.45
-3.02
1.01
-3.66
-4.06
209747_at
TGFβ3
-4.99
-1.22
3.16
3.22
2.22
2.18
3.01
206943_at
TGFβRI
4.03
-2.99
-3.05
-4.01
-2.1
-2.62
-2.66
207334_s_at
TGFβRII
4.15
-3.21
-3.33
-2.96
-4.36
-4.69
-5.02
204731_at
TGFβRIII
4.18
1.85
-1.74
-1.72
-1.11
-1.22
-1.31
206675_s_at
SKIL
-7.26
-4.02
2.23
3.11
-5.22
1.11
2.01
205596_s_a
SMURF2
7.09
-2.11
-9.14
-9.19
-3.39
-10.21
12.01
205397_x_at
SMAD3
18.96
-7.01
-11.99
-15.03
-8.13
19.11
-20.94
205289_at
BMP2
-11.55
8.01
7.96
8.12
3.62
7.05
7.06
206176_at
BMP6
-14.02
+1.01
19.06
22.05
-2.24
2.09
6.33
205842_s_at
JAK2
4.36
-1.25
-1.98
-2.06
-2.22
-4.08
-4.01
214590_s_at
UBE2D1
-3.88
-1.11
3.08
3.14
-2
1.11
-1.33
210567_s_at
SKP2
5.02
2.62
-3.42
-3.22
1.79
-2.33
-1.17
218995_s_at
EDN1
4.14
-1.48
-1.95
-2.07
-2.03
-1.99
-2.74
203680_at
PRKAR2B
4.29
1.22
-1.5
-1.11
-1.22
-1.32
-1.18
+ indicates overexpression of gene (increased level of mRNAs); - indicates suppressed gene expression (decreased level of mRNAs); ID: ID of the probe on a microarray; FC: fold change; C: control culture; H-2, H-8, and H-24: time of exposure to the medicine.
