IL-33 Mediates Lung Inflammation by the IL-6-Type Cytokine Oncostatin M

Figure 3

IL-33 is required for AdOSM-mediated lung inflammation. C57Bl/6 mice were endotracheally administered AdDl70 (control) or AdOSM and analyzed after 7 or 14 days. Representative H&E lung sections are shown from two different mice. (a) Wild-type (WT; top) or IL-33-/- (bottom) mice infected with AdDl70. (b) Wild-type (WT; top) or IL-33-/- (bottom) mice infected with AdOSM. (c) Total cells collected from bronchoalveolar lavage (BAL) fluid from C57Bl/6 mice at Day 7 (c) or Day 14 (d). Hema-3 staining of cytospin slides of BAL cells was done to distinguish neutrophils, eosinophil, and macrophage cell populations. , , . . (e) Representative images of arginase-1 (Arg1) immunohistochemistry staining (brown arrows) of lung sections from C57Bl/6 wild-type and IL-33-/- mice treated as described in (a).

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