Monocytes Undergo Functional Reprogramming to Generate Immunosuppression through HIF-1α Signaling Pathway in the Late Phase of Sepsis

Figure 6

Its immunofluorescence analysis of proteins HIF-1α and PD-L1 (200x) in the clinical study. (a) The protein HIF-1α (red) is located in the nuclear, the immune checkpoint protein PD-L1 (green) is expressed on the monocyte membrane, and DAPI was used to stain the nucleus (blue). (b) Reducing the mean fluorescence intensity (MFI) of protein HIF-1α in the late phase of sepsis, of HIF-1α and PD-L1 versus the normal control group represented significant difference, while increasing the fluorescence intensity (MFI) of protein PD-L1 in the late phase of sepsis versus the early phase, ^# of PD-L1 versus normal control group represented significant difference.

(a)

(b)