IκB Kinase Inhibitor VII Modulates Sepsis-Induced Excessive Inflammation and Cardiac Dysfunction in 5/6 Nephrectomized Mice

Figure 8

Blockade of IKK provides attenuation and enhances the phosphorylation of IKKα/β and IkBα nuclear translocation of NF-κB and levels of iNOS in 5/6 nephrectomized CLP mice. The 5/6 nephrectomized mice were subjected to sham surgery, and 5/6 nephrectomized mice were subjected to CLP or sham surgery; after 1 hour post-CLP and 5/6 nephrectomy, the mice were treated with IKK inhibitor VII or vehicle (DMSO). The signaling effects in cardiac tissues were analyzed after 24 hours. Optical density of bands was assessed for the following: (a) phosphorylation of IKKα/β expressed to respective total IKKα/β normalized to respective sham band; (b) optical density of phosphorylated IkBα against total IkBα levels normalized against corresponding sham band; (c) levels of NF-κB in nuclear and cytosolic fractions presented as nucleus/cytosol ratio; the results were normalized against corresponding sham band; (d) levels of iNOS normalized against actin as loading control. Each experiment was done in triplicate; the data are presented as . compared to 5/6 nephrectomized mice, CLP mice, and vehicle-treated group.

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