Global Transcriptional Profiling Reveals Novel Autocrine Functions of Interleukin 6 in Human Vascular Endothelial Cells

Figure 4

Knockdown of IL6 in human endothelial cells affects the gene expression of proteins known to be involved in IL6-mediated signaling. Knockdown of IL6 was performed using siRNA-mediated gene silencing followed by RNA sequencing in cultured human vascular endothelial cells. The impact of IL6 knockdown on genes known to be central in IL6 signaling (IL6 core genes) was investigated. Green symbols indicate downregulated genes, and red/pink symbols indicate upregulated genes. Pathway and coloring were generated using Ingenuity Pathway Analysis software.