High T3 Induces β-Cell Insulin Resistance via Endoplasmic Reticulum Stress
Expression of p-IRS-1ser307, Akt, and insulin in β-cells treated with different concentrations of T3. N group: normal-concentration T3 (5 pg/mL); H group: high-concentration T3 (20 pg/mL); H+SV group: high-concentration T3 (20 pg/mL)+sodium orthovanadate (5 μmol/L). (a) Western blotting analysis showed that the expression of p-IRS-1ser307 in the H group was significantly higher than that in the N group, whereas Akt was lower. (b) p-IRS-1ser307 in the H+SV group was significantly lower than that in the H group, whereas Akt was higher than that in the H group. (c) ELISA showed that the concentration of insulin in the supernatant of the N group was significantly higher than that of the H group, whereas that of the H group was significantly lower than that of the H+SV group (,,,). ELISA: enzyme-linked immunosorbent assay.
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