High T3 Induces β-Cell Insulin Resistance via Endoplasmic Reticulum Stress
Expression of specific proteins of ER and insulin in β-cells treated with different concentrations of T3. N group: normal-concentration T3 (5 pg/mL); H-group: high-concentration T3 (20 pg/mL); H+4-PBA group: high-concentration T3 (20 pg/mL)+4-PBA (1 mmol/L). (a) Western blotting analysis showed that the expression of GRP78, PERK, IRE1, and ATF6 in the H group was significantly higher than that in the N group. (b) The expression of GRP78, PERK, IRE1, and ATF6 in the H+4-PBA group was significantly lower than that in the H group. (c) The expression of p-IRS-1ser307 in the H+4-PBA was significantly lower than that in the H group, whereas Akt expression was higher than that in the H group. (d) ELISA showed that the concentration of insulin in the supernatant of the H group was significantly lower than that of the H+4-PBA group (,,). ELISA: enzyme-linked immunosorbent assay.
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