Research Article
PDK1/mTOR Signaling in Myeloid Cells Differentially Regulates the Early and Late Stages of Sepsis
Figure 3
Myeloid-specific deletion of mTOR core factors Raptor and Rictor aggravated CLP-induced sepsis. (a) Littermate Raptorfl/fl and Raptorfl/fl/LyzM-Cre mice were subjected to CLP operation, and the survival time was monitored 5 days after the operation. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (b–e) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Raptorfl/fl and Raptorfl/fl/LyzM-Cre mice at 12 h, 24 h, and 48 h after CLP. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; . (f) Littermate Rictorfl/fl and Rictorfl/fl/LyzM-Cre mice were subjected to CLP operation, and the survival time was monitored 5 days after the operation. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (g–j) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Rictorfl/fl and Rictorfl/fl/LyzM-Cre mice at 12 h, 24 h, and 48 h after CLP. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; .
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