PDK1/mTOR Signaling in Myeloid Cells Differentially Regulates the Early and Late Stages of Sepsis

Figure 4

Myeloid-specific PDK1 deletion tuned down LPS-induced inflammation in the later stage of sepsis. (a) Littermate PDK1^fl/fl and PDK1^fl/fl/LyzM-Cre mice were subjected to CLP operation, and 7 days after operation, LPS (10 mg/kg) was peritoneally injected, and the survival time was monitored 5 days after the injection. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (b–e) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of PDK1^fl/fl and PDK1^fl/fl/LyzM-Cre mice at 6 h, 12 h, and 24 h after LPS injection. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; . The diagram on the bottom part of the figure was the CLP later stage experimental protocol. PDK1^fl/fl and PDK1^fl/fl/LyzM-Cre mice received the CLP procedure, and 7 days after CLP, LPS was injected to mice. The survival time of all the mice was monitored until 5 days after the LPS injection. The cytokines were collected after LPS injection in the indicated time.