PDK1/mTOR Signaling in Myeloid Cells Differentially Regulates the Early and Late Stages of Sepsis

Figure 5

Myeloid-specific Raptor and Rictor deletion tuned down LPS-induced inflammation in the later stage of sepsis. (a) Littermate Raptor^fl/fl and Raptor^fl/fl/LyzM-Cre mice were subjected to CLP operation, and 7 days after operation, LPS (10 mg/kg) was peritoneally injected, and the survival time was monitored 5 days after the injection. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (b–e) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Raptor^fl/fl and Raptor^fl/fl/LyzM-Cre mice at 6 h, 12 h, and 24 h after LPS injection. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. ; . (f) Littermate Rictor^fl/fl and Rictor^fl/fl/LyzM-Cre mice were subjected to CLP operation, and 7 days after operation, LPS (10 mg/kg) was peritoneally injected, and the survival time was monitored 5 days after the injection. The comparison of survival in different mice was calculated by the Kaplan-Meier method (). (b–e) The measurement of TNFα, IL-6, IL-1β, and IFN-γ secretion by ELISA from serum of Rictor^fl/fl and Rictor^fl/fl/LyzM-Cre mice at 6 h, 12 h, and 24 h after LPS injection. The comparison of cytokine levels in different mice was calculated by the Mann–Whitney test. , , and .

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