Baicalein Restores the Balance of Th17/Treg Cells via Aryl Hydrocarbon Receptor to Attenuate Colitis

Figure 1

Baicalein activated AhR in EL-4 cells. (a) The viability and proliferation of EL-4 cells were detected by using MTT assays. (b–f) EL-4 cells were treated with baicalein (25 μM and 50 μM), TCDD (5 nM), CH223191 (10 μM), and CH223191 (10 μM)+baicalein (50 μM) for 24 h; nuclear and cytoplasmic proteins of AhR and CYP1A1 were detected by using western blotting (WB), respectively. (g) The nuclear translocation of AhR was detected by using immunofluorescence (scale bar: 4 μm). Experiments were repeated three times, and the results were expressed as ; and vs. control model. CH: CH223191; CH+B: CH223191+baicalein.

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