Phenotypic characterization of lymphocyte subpopulations by flow cytometry. (a) Representative flow cytometry analysis of peripheral lymphocytes. T: CD45+ CD3+; B: CD45+ CD3- CD19+; NK: CD45+ CD3- CD16+ CD56+; CD4+ T: CD45+ CD3+ CD4+; CD8+ T: CD45+ CD3+ CD8+. (b) Representative flow cytometry analysis of CD4+ T cell subsets. All dot plot analysis is of CD4+ gated lymphocyte. Th1:CD4+ INF-γ+; Th2: CD4+ IL-4+; Th17: CD4+ IL-17+; Treg: CD4+ CD25+ Foxp3+.