Research Article

Inhibition of miR-17~92 Cluster Ameliorates High Glucose-Induced Podocyte Damage

Figure 2

The effect of miR-17∼92 cluster silence on cell viability, apoptosis, and inflammation of HG-treated MPC5 cells. (a) The expression levels of miR-17∼92 cluster members in MPC5 cells. (b, c) Cell viability (b) and apoptosis (c) of HG-treated MPC5 cells were detected through CCK-8 assay and Annexin V/PI double stain assay. (d, e) The content of IL-6 (d) and TNF-α (e) in the culture medium of MPC5 cells were measured through ELISA kits. Cells were transfected with antagomirs of the miR-17∼92 cluster members (Anta-miR-17∼92) or antagomirs with negative control sequence (Anta-NC) and cultured for 48 h; or then, the normal culture medium (glucose, 5 mM) was replaced by high glucose-containing medium (glucose, 25 mM), cultured for 24 h. , vs. the control group; #, vs. the HG+Anta-NC group.