Aloe Emodin Reduces Cardiac Inflammation Induced by a High-Fat Diet through the TLR4 Signaling Pathway

Figure 4

AE has an important repressor effect on the HFD-stimulated TLR4 pathway signaling. 3D schematic diagram of interaction between AE and TLR4 active site residues: the yellow dotted line is hydrogen bonding (a). Heart tissue homogenate was extracted for western blot assay. GAPDH was used as a loading control. The column figures have shown the normalized optical density of each protein as follows: (b) Toll-like receptor 4 (TLR4), (c) inhibitor of NF-κB (IκB), (d) nuclear factor-kappa B P65 (NF-κB P65), and (e) p-nuclear factor- kappa B P65 (p-NF-κB P-P65) (; and compared to the ND group; ^#, ^##, and ^### compared to the HFD group).

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