Nicotine Impairs the Response of Lung Epithelial Cells to IL-22
AHR ligand, FICZ, induces IL-22 production in PBMCs from healthy subjects which is suppressed by nicotine. Anti-CD3 and CD28 bead-stimulated PBMCs were exposed to FICZ, nicotine, or both for 5 days. (a) ELISA for IFN-γ and IL-22. Result is presented as the of 4 experiments. indicates that value is significant. (b) Gated CD4 T cells were analyzed for IL-22 expression by intracellular cytokine staining (ICC) using flow cytometry. Green dot plots depict the gating strategy. PBMCs without bead stimulation are shown as controls. Data is representative of 4 experiments.
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