Dexmedetomidine Attenuates Monocyte-Endothelial Adherence via Inhibiting Connexin43 on Vascular Endothelial Cells

Figure 2

Inhibiting Cx43 on HUVECs could attenuate U937-HUVEC adhesion via modulating the MAPK signaling pathway. (a) Effects of two kinds of Cx43-siRNAs on the activation of MAPKs (p-ERK/ERK, p-p38/p38, and p-JNK/JNK) (, vs. control). Control group means lipofectamine 2000 pretreatment group; NC: negative control (negative control has random base sequence that differs from Cx43-siRNA1 and Cx43-siRNA1). (b–d) The contents of MCP-1, sICAM-1, and sVCAM-1 when HUVECs were pretreated with U0126 (inhibiting p-ERK1/2, 10 μM, 24 hours), SB202190 (inhibiting p38, 10 μM, 24 hours), and SP600125 (inhibiting p-JNK1/2, 10 μM, 24 hours) (, vs. control). (e) The changes of U937-HUVEC adhesion when HUVECs were pretreated with U0126, SB202190, and SP600125 (, vs. control). The solvents of U0126, SB202190, and SP600125 were DMSO, which had no effects on the above results. (b–e) The control group is just DMSO pretreatment. In all experiments, HUVECs were all pretreated with TNF-α (10 ng/ml, 12 h). Effects of TNF-α are showed in Supplemental Figure 1.

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