Suppressors of Cytokine Signaling (SOCS)1 and SOCS3 Proteins Are Mediators of Interleukin-10 Modulation of Inflammatory Responses Induced by Chlamydia muridarum and Its Major Outer Membrane Protein (MOMP) in Mouse J774 Macrophages

Figure 1

MOMP is a potent inducer of inflammatory mediators that are dose-dependently inhibited by added IL-10 to macrophages. For chlamydial stimulant dose-dependent studies, macrophages (10⁶ cells/mL) were stimulated with rMOMP at 0.1, 1 and 10 μg/mL (A-D) or live Cm at MOIs of 0.5, 1 and 2 (E-H) in the presence and absence of IL-10 (10 ng/mL). For IL-10 dose-dependent studies, macrophages were stimulated with rMOMP at 10 μg/mL (I-L) or Cm at an MOI of 2 (M-P) in the presence and absence of varying dosages of IL-10 (0.1, 1 and 10 ng/mL). Cell-free culture supernatants were collected after 24 h to quantify cytokines and chemokines by specific ELISAs. Asterisks indicate significant differences between stimulated macrophages alone and those with added IL-10 (P <0.05). P values were calculated by the use of ANOVA followed by Turkey’s Post-test using GraphPad Prism 6 Software. Each bar represents the mean ± SD of samples run in triplicates. Each experiment was repeated at least 4 times with similar results and shown is a representative experiment.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)

(k)

(l)

(m)

(n)

(o)

(p)