Suppressors of Cytokine Signaling (SOCS)1 and SOCS3 Proteins Are Mediators of Interleukin-10 Modulation of Inflammatory Responses Induced by Chlamydia muridarum and Its Major Outer Membrane Protein (MOMP) in Mouse J774 Macrophages

Figure 8

Inhibition of proteasomal degradation alters the IL-10 inhibition of inflammatory mediators and the transcriptional activation of SOCS and STATs in chlamydial macrophage cultures. Macrophages (10⁶/mL) were treated with 0, 1, and 20 nM of Bortezomib (Btzb) for 1 h, followed by stimulation with rMOMP (1 μg/mL) or Cm (MOI of 2) in the presence and absence of IL-10 for 24 h. Post stimulation, cell-free culture supernatants were collected to quantify TNF (A), IL-10 (B), CCL5 (C) and CXCL10 (D) by specific ELISAs. The mRNA gene transcripts for socs1 and socs3 (E) and stat1 and stat3 (F) were quantified via TaqMan qRT-PCR. An asterisk indicates a significant difference (P <0.05), and P values were calculated as described in Figure 1 legend. Each bar represents the mean ± SD of samples run in triplicates. Each experiment was repeated at least 3 times.

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