Expression of Matrix Metalloproteinases and Their Tissue Inhibitors in Peripheral Blood Leukocytes and Plasma of Children with Nonalcoholic Fatty Liver Disease

Table 2

Primer sequence of target genes and reference gene for SYBR Green real-time PCR.

Gene	Forward primer	Reverse primer
MMP-9	CAA CAT CAC CTA TTG GAT CC	CGG GTG TAG AGT CTC TCG CT
MMP-2	TGA TCT TGA CCA GAA TAC CAT CGA	GGC TTG CGA GGG AAG AAG TT
TIMP-1	CTT CTG GCA TCC TGT TGT TG	AGA AGG CCG TCT GTG GGT
TIMP-2	CGA CAT TTA TGG CAA CCC TAT CA	CAG GCC CTT TGA ACA TCT TTA TCT
MMP-12	TTCCCCTGAACAGCTCTACAAGCCTGGAAA	GATCCAGGTCCAAAAGCATGGGCTAGGATT
MMP-14	CGC TAC GCC ATC CAG GGT CTC AAA	CGC TAC GCC ATC CAG GGT CTC AAA
TGF-β	GGA AAC CCA CAA CGA AAT CTA TG	CGG GTT CAG GTA CCG CTT C
IL-6	TGA AAG CAG CAA AGA GGC ACT	GGC AAG TCT CCT CAT TGA ATC C
G3PDH	GCG GGG CTC TCC AGA ACA TCA T	CCA GCC CCA GCG TCA AAG GTG

MMP-2, 9, 12, and 14 indicate matrix metalloproteinase-2, 9, 12, or 14, respectively. TIMP-1 and 2 indicate tissue inhibitor of metalloproteinase-1 or 2. TGF-β: transforming growth factor beta; IL-6: interleukin-6; G3PDH: glyceraldehyde-3-phosphate dehydrogenase.