Effects of Urate-Lowering Therapy on Risk of Hyperlipidemia in Gout by a Population-Based Cohort Study and on In Vitro Hepatic Lipogenesis-Related Gene Expression

Figure 3

Expression of hepatic lipid metabolism-related genes following treatment with T0901317 and antigout drugs. Differentiated HepaRG cells were treated for 24 h with T0901317 (10 μM), allopurinol (14.7 and 22.1 μM), febuxostat (5.66 and 13.09 μM), benzbromarone (4.24 and 7.73 μM), sulfinpyrazone (32.24 and 56.04 μM), probenecid (243.9 and 520.6 μM), and colchicine (^-4 and 0.075 μM). Following treatment, RNA was extracted, and the expression levels of (a) SREBP-1c, (b) SCD, (c) FAS, (d) FAE, (e) ACLY, (f) ACC, and (g) LXRα were analyzed by quantitative reverse transcription-polymerase chain reaction. Values were normalized to the expression of β-actin, with the β-actin levels of dimethyl sulfoxide- (DMSO-) treated cells set at 1. Results are expressed as the (SE) (). , , and compared with cells treated with DMSO. SREBP-1c: sterol regulatory element binding protein 1; SCD: stearoyl-CoA desaturase-1; FAS: fatty acid synthase; FAE: fatty acid elongase; ACLY: adenosine 5-triphosphate (ATP) citrate lyase; ACC: acetyl-CoA carboxylase; LXRα: liver X receptor alpha.

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