Mediators of Inflammation / 2020 / Article / Fig 4

Research Article

Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways

Figure 4

Luteolin inhibited NF-κB activation and thereby suppressed THP-1 cell adherence to IL-1β-stimulated ARPE-19 cells. Cells were pretreated with different doses of luteolin (LU) and then incubated with IL-1β (1 ng/mL) for 15 min. NF-κB p65 translocation was evaluated by immunofluorescence staining. Green: location of the p65 subunit. Blue: DAPI for nuclear staining. (a) DAPI staining revealed that luteolin attenuated NF-κB p65 translocation in IL-1β-stimulated ARPE-19 cells. (b) Western blots show the levels of phosphorylated IκB protein expression. (c) Luteolin suppressed THP-1 adhesion. ARPE-19 cells were pretreated with 10 μM luteolin or 5 μM Bay 117082 for 1 h and then cocultured with labeled THP-1 cells. (d) Fluorescence intensity showed THP-1 cell adhesion to IL-1β-stimulated ARPE-19 cells, which was used to quantify calcein-AM fluorescence. Data represent the . , compared to ARPE-19 cells stimulated with IL-1β alone.
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