Mediators of Inflammation / 2020 / Article / Fig 5

Research Article

Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways

Figure 5

Luteolin blocked JNK phosphorylation, and the JNK inhibitor SP60012 (SP) decreased THP-1 cell adherence to IL-1β-stimulated ARPE-19 cells. (a) Western blots show the levels of phosphorylated JNK protein expression. (b) The fold-change in pJNK protein expression was measured relative to JNK expression. (c) ARPE-19 cells were pretreated with 10 μM luteolin or JNK inhibitor (SP600125) for 1 h and then cocultured with labeled THP-1 cells. (d) The fluorescence intensity was used to quantify calcein-AM fluorescence. Data represent the . , , compared to ARPE-19 cells stimulated with IL-1β alone.

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