Mediators of Inflammation / 2020 / Article / Fig 6

Research Article

Luteolin Attenuates IL-1β-Induced THP-1 Adhesion to ARPE-19 Cells via Suppression of NF-κB and MAPK Pathways

Figure 6

Luteolin blocked ERK phosphorylation and the ERK inhibitor PD98059 (PD) decreased THP-1 cell adherence to IL-1β-stimulated ARPE-19 cells. (a) Western blots show levels of phosphorylated ERK protein expression. (b) The fold-change in pERK protein expression was measured relative to ERK expression. (c) ARPE-19 cells were pretreated with 10 μM luteolin or ERK inhibitor (PD98059) for 1 h and then cocultured with labeled THP-1 cells. (d) The fluorescence intensity was used to quantify calcein-AM fluorescence. Data represent the . , , compared to ARPE-19 cells stimulated with IL-1β alone.

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.