Mediators of Inflammation / 2020 / Article / Fig 3

Research Article

lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis

Figure 3

lncRNA IGHCγ1 functioned as ceRNA via binding miR-6891-3p. (a) Real-time PCR revealed lncRNA IGHCγ1 primarily existed in the cytoplasm of macrophages (these data represented 3 independent experiments; compared with controls, ; compared with the LPS-treated macrophage group, ###). (b) FISH also showed lncRNA IGHCγ1 was mainly expressed in pTHP-1 cytoplasm (pictures represent one of three repeated FISH assays). (c) Increased copy number gains of lncRNA IGHCγ1 in OA PBMC samples (). (d) Increased copy number gains of lncRNA IGHCγ1 relative to miR-6891-3p in pTHP-1 cells stimulated by LPS (; ; ). (e) As shown by real-time PCR, the expression of miR-6891-3p in macrophages was significantly reduced when lncRNA IGHCγ1 was overexpressed (; ). (f) The seed sequence of miR-6891-3p recognized by lncRNA IGHCγ1 (data were screened in database of starBase). (g) Decreased luciferase activity in lncRNA IGHCγ1 WT transfected cells but not lncRNA IGHCγ1 MT cells (; ). (h) RIP assay showed IGHCγ1 in immunoprecipitates. Cell lysates were immunoprecipitated by use of Ago2 antibody and IgG. IGHCγ1 expression is determined by real-time PCR (; ).

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