Mediators of Inflammation / 2020 / Article / Fig 7

Research Article

lncRNA IGHCγ1 Acts as a ceRNA to Regulate Macrophage Inflammation via the miR-6891-3p/TLR4 Axis in Osteoarthritis

Figure 7

lncRNA IGHCγ1 functioned as a ceRNA for miR-6891-3p through NF-κB signaling. (a) lncRNA IGHCγ1 promoted NF-κB phosphorylation by sponging miR-6891-3p, while miR-6891-3p mimics rescued its inhibitory effect on NF-κB phosphorylation in macrophages (representative western blot assay). (b) The densitometry of p-NF-κB determined by western blot (; in contrast to the -/- group, ; compared with the +/- group, #). (c) lncRNA IGHCγ1 increased NF-κB activity by acting as ceRNA for miR-6891-3p, whereas miR-6891-3p mimics rescued its effect on NF-κB in pTHP-1 cells (; ). (d) Expression of IL-6 and TNF-α mRNAs in macrophages (; compared with the control (-/-) group, ; in contrast to the lncRNA/mimics (+/-) group, #; ##). (e) IL-6 and TNF-α in the supernatant of macrophages (; in contrast to the control (-/-) group, ; compared with the lncRNA/mimics (+/-) group, ##).

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